Reversed phase planar chromatography of isomers using α- and beta-cyclodextrin solutions as eluents
Summary
Type: TLC Application #: 400850
Detection: Eluent A: 2.5 g NaCl + 26 g urea in water, -
dissolved to 100 ml
30 ml methanol
Eluent B: eluent A containing 0.05 M b-cyclodextrin
Detection: UV, 254 nm Conditions: Eluent for A: 2.5 g NaCl + 5 g urea in water, -dissolved to 100 ml 30 ml methanol 0.1 M a-cyclodextrin Eluent for B: 2.5 g NaCl + 26 g urea in water, -dissolved to 100 ml 30 ml methanol 0.1 M b-cyclodextrin Eluent C: eluent A containing 0.1 M b-cyclodextrin Eluent D: 2.5 g NaCl + 5 g urea in water dissolved to 100 ml 30 ml methanol 0.1 M a-cyclodextrin Sample volume: 0.2 – 0.5 ul of a 1 mg/ml sample in water / acetonitrile (40/60) and cyclohexane Migration distance: 8 cm Authors: Lepri L Source: J Planar Chromatog 4 (1991) 338-340